Tips on how to get the most out of Vysis products. These might range from simple tips on maximizing the performance of Vysis Probe and instrument products, to detailed protocols for using Vysis products in your applications. Check here first for the most current technical information. Don't forget about the Vysis FAQs; where you'll find the answers to many questions frequently asked of our Technical Service staff.
Controls and CGH
When performing CGH experiments, the proper use of controls cannot be overemphasized. Controls should be run to verify that your labeling reaction was efficient and additional controls should be run to verify that your hybridization was efficient. Also, each time DNA is isolated, a gel should be run to verify that the DNA you are trying to label is of the optimal fragment size and that it is clean. If any of these steps is omitted and your end results are less than optimal, it will be very difficult to determine exactly what went wrong and what should be done to correct it in the next experiment. For further information on the proper use of controls, you may contact Vysis® technical service.
Lot Numbers Explained
The lot number that appears on the Certificate of Analysis that is furnished with each probe is the kit lot number. Most kits are comprised of a vial of probe and a vial of hybridization buffer. Other kits contain probe pre-mixed with hybridization buffer. Each of the component parts has a unique part number and lot number separate from those of the kit. When you order the product from our catalog, the number you use is the catalog number, not the individual part numbers that appear on the vials contained within the kit.
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