The unique designs of the Vysis DNA FISH probes provide distinct advantages for detection of critical genetic aberrations associated with hematopoietic disorders. The unique types of Vysis DNA FISH probe designs for chromosome translocations involved in these disorders are described below:
Dual Color, Single Fusion
Useful for detecting high percentages of cells possessing a specific chromosomal translocation. The DNA probe hybridization targets are located on one side of each of the two genetic breakpoints. ES (Extra Signal) reduces the frequency of normal cells exhibiting an abnormal FISH pattern due to the random co-localization of probe signals in a normal nucleus. One large probe spans one breakpoint, while the other probe flanks the breakpoint on the other gene.
Dual Color, Break Apart
Useful in cases where there may be multiple translocation partners associated with a known genetic break-point. This labeling scheme features two differently colored probes which hybridize to targets on opposite sides of a breakpoint in one gene.
Dual Color, Dual Fusion
Greatly reduces the number of normal nuclei exhibiting abnormal signal patterns. Optimal for detecting low levels of nuclei possessing a simple balanced translocation. Large probes span two breakpoints on different chromosomes.