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In most cases, regardless of the cause, if inadequate FISH results are obtained, slides can be re-hybridized with a new probe mixture. If the probe is direct-labeled, then the same or a different probe can be used.
Conditions are based on peripheral blood samples.
The counterstain intensity decreases with sequential hybridizations, however, the loss is minimal. It is possible to perform up to 8 repeated hybridizations of the same metaphase with different probes.
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