Sequential FISH Procedure

In most cases, regardless of the cause, if inadequate FISH results are obtained, slides can be re-hybridized with a new probe mixture. If the probe is direct-labeled, then the same or a different probe can be used.  

Conditions are based on peripheral blood samples.

  1. Using forceps, carefully remove the coverslip from the previously hybridized targets.
  2. Dehydrate the slides at room temperature by immersing the slide in aseries of ethanol wash solutions of 70%, 85%, and 100% for 1 minuteeach. Allow the slide to air dry. Slides are ready for repeat hybridization. It is recommended that the slide denaturation time bedecreased to approximately 50% of the original denaturation time, but not less than 3 minutes. Subsequent re-hybridization does not requirefurther reduction of the denaturation time.

The counterstain intensity decreases with sequential hybridizations, however, the loss is minimal. It is possible to perform up to 8 repeated hybridizations of the same metaphase with different probes.

You are about to exit the Abbott family of websites for a 3rd party website

Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.

Do you wish to continue and exit this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No