Short version protocol should not replace the package insert.
To prepare paraffin-embedded tissue sections fixed on positively charged slides for use in fluorescence in situ hybridization (FISH*) with Vysis Multi-color DNA FISH probes.
Summary and Principle
The procedure that follows has been designed to maximize tissue processibility and permeability for FISH when using Vysis Multi-color DNA FISH probes (e.g., ProVysionTM Multi-colorProbe, Order No. 32-131090). This pretreatment assay will help minimize heavy particulate background after DNA probe hybridization, which may sometimes occur in specific tissue types.
Sample Slides Preparation
Note: Use samples that were fixed in formalin for between 24-48 hours.
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