- Your Challenges
- News & Events
- About Us
To prepare paraffin-embedded tissue sections fixed on positively charged slides for use in fluorescence in situ hybridization (FISH) with Vysis Multi-color DNA FISH probes.
Summary and Principle
The procedure that follows has been designed to maximize tissue permeability for FISH when using Vysis Multi-color DNA FISH probes.
Reagents Provided in Kit Kit Order #32-801210 For Laboratory Use
|Pretreatment Solution||5 x 50 mL||Sodium Thiocyanate (NaSCN)||2 to 25 °C|
|Protease Buffer II||5 x 62.5 mL||0.2 N HCl, pH 1.0||2 to 25 °C|
|Protease||5 x 250 mg||2500 - 3000 U/mg protease, lyophilized||-20 to 8 °C|
Materials Required but not Provided
Sample Slides Preparation
Note: Use samples that were fixed in formalin for between 24-48 hours.
Fixing the Sample
Note: Fixation of the sample is performed to minimize tissue loss during sample denaturation. This procedure is highly recommended when processing samples in a denaturatrion bath format.
Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.
Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.