To prepare paraffin-embedded tissue sections fixed on positively charged slides for use in fluorescence in situ hybridization (FISH) with Vysis Multi-color DNA FISH probes.
Summary and Principle
The procedure that follows has been designed to maximize tissue permeability for FISH when using Vysis Multi-color DNA FISH probes.
Reagents Provided in Kit Kit Order #32-801210 For Laboratory Use
|Pretreatment Solution||5 x 50 mL||Sodium Thiocyanate (NaSCN)||2 to 25 °C|
|Protease Buffer II||5 x 62.5 mL||0.2 N HCl, pH 1.0||2 to 25 °C|
|Protease||5 x 250 mg||2500 - 3000 U/mg protease, lyophilized||-20 to 8 °C|
Materials Required but not Provided
Sample Slides Preparation
Note: Use samples that were fixed in formalin for between 24-48 hours.
Fixing the Sample
Note: Fixation of the sample is performed to minimize tissue loss during sample denaturation. This procedure is highly recommended when processing samples in a denaturatrion bath format.
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