To prepare paraffin-embedded tissue sections fixed on positively charged slides for use in fluorescence in situ hybridization (FISH) with Vysis probes.
|Pretreatment Solution||5 x 50 mL||Sodium Thiocyanate (NaSCN)||2 to 25 °C|
|Protease Buffer||5 x 50 mL||NaCl, pH 2.0||2 to 25 °C|
|Wash Buffer||5 x 250 mg||2X SSC, pH 7.0||2 to 25 °C|
|Protease||5 x 25 mg||2500 - 3000 U/mg protease, lyophilized||-20 °C|
Preparing Specimen Slides (optional)
Note: Use specimens that were fixed in formalin for between 24-48 hours.
Treating Slides With Protease
Fixing the Specimen
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