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Intended Use
To prepare paraffin-embedded tissue sections fixed on positively charged slides for use in fluorescence in situ hybridization (FISH) with Vysis probes.
Reagents Provided
Reagent | Quantity | Composition | Storage |
Pretreatment Solution | 5 x 50 mL | Sodium Thiocyanate (NaSCN) | 2 to 25 °C |
Protease Buffer | 5 x 50 mL | NaCl, pH 2.0 | 2 to 25 °C |
Wash Buffer | 5 x 250 mg | 2X SSC, pH 7.0 | 2 to 25 °C |
Protease | 5 x 25 mg | 2500 - 3000 U/mg protease, lyophilized | -20 °C |
Preparing Specimen Slides (optional)
Note: Use specimens that were fixed in formalin for between 24-48 hours.
Deparaffinizing Slides
Pretreating Slides
Treating Slides With Protease
Fixing the Specimen
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