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for use in FISH slide preparation ...
This method produces chromosomes with a band resolution of 400-450 and is sufficient for most FISH applications.
for use in CGH slide preparation ...
These additional steps increase chromosome elongation and mitotic index. For CGH, the chromosomes should have a band resolution of 400-550.
|
for use in FISH slide preparation ...
This method produces chromosomes with a band resolution of 400-450 and is sufficient for most FISH applications.
for use in CGH slide preparation ...
These additional steps increase chromosome elongation and mitotic index. For CGH, the chromosomes should have a band resolution of 400-550.
|
Note: You add fixative to the cell pellet until the suspension is slightly cloudy; the amount of fixative needed will depend on the size of the cell pellet.
Cells may be stored at 4 °C in tightly-capped centrifuge tube until ready touse. If slides will not be prepared within 7 DAYS, bring the total volume to 10 mL and store at -20 °C. Repeat the wash described in steps8a-8e two times prior to preparing the slides.
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