Frozen Tissue Prep for FISH

Standard Protocol

  • Cut 5um sections on silanized or positively charged slides
  • Dry at room temperature for 15-30 min
  • Fix in 4% formaldehyde in PBS at 0 ºC for 15 min
  • Wash with PBS and start the Paraffin Pretreatment kit protocol at the .2N HCl step and follow through to the end

Alternate Protocol

  • Cut sections as before and dry
  • Fix in Carnoy's fixative (3:1 methanol: acetic acid) for 5 min.
  • Begin the Paraffin Pretreatment kit protocol just prior to the Protease step (Wash Buffer for 5 min.)

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