- Cut 5um sections on silanized or positively charged slides
- Dry at room temperature for 15-30 min
- Fix in 4% formaldehyde in PBS at 0 ºC for 15 min
- Wash with PBS and start the Paraffin Pretreatment kit protocol at the .2N HCl step and follow through to the end
- Cut sections as before and dry
- Fix in Carnoy's fixative (3:1 methanol: acetic acid) for 5 min.
- Begin the Paraffin Pretreatment kit protocol just prior to the Protease step (Wash Buffer for 5 min.)