Short version protocol should not replace the package insert.
To prepare cells fixed onto slides for use in fluorescence in situ hybridization (FISH) with Vysis probes.
|Pepsin Buffer||3 x 50 mL||10 mM HCl||2 to 25 °C|
|Protease||3 x 25 mg||2500 - 3000 U/mg protease, lyophilized||-20 to 8 °C|
|PBS||2 x 250 mL||1X PBS||2 to 25 °C|
|100X MgCl2||3 x 0.5 mL||2M MgCl2 * 6 H2O||2 to 25 °C|
|20X SSC||1 x 66g||Sodium Chloride & Sodium Citrate||-25 to 30 °C|
Materials Required but not Provided
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