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Interpretation of Results
Enumeration guidelines for CEP and LSI on interphase nuclei are indicated below. The user is encouraged to apply these guidelines and provide additional guidelines as necessary to improve enumeration precision, accuracy and reproducibility within the laboratory. A slide scanning technique for CEP® and LSI® enumeration is described below. This technique or another, provided it is standardized in the lab, may increase enumeration accuracy and reproducibility.
Recommended Enumeration Guidelines for Single Color Probe Enumeration
Single color probe enumeration can be performed using a single bandpassfilter, or muti-bandpass filter set in order to visualize the counterstain fluorescence (if present).
Two or more probes labeled with different color fluorophores that are hybridized to the same cells may be enumerated singly using single bandpass filter sets or simultaneously using multi-bandpass filter sets depending upon the signal intensity of the probes and the size of the nucleus. Cells with small or constricted nuclei may not be easily enumerated using multi-bandpass filter sets due to the spacial arrangement of the probe signal in the nucleus or due to perceived dim probe signals.
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