Microscope Filters

microscope filters

 

Microscope Filter Order Form »

categoryimages-fishmicro1.jpg

 

The Vysis microscope filter sets are 

high-quality filter sets that are designed to provide optimal excitation and emission of the Vysis fluorophore labeled DNA FISH probes. The specific Vysis design filter sets provide a wider bandpass, steeper profile, and maximum fluorescence throughput specific for Vysis fluorophores. In addition, the Vysis filter sets carry a one year warranty against defects.

 

Many of the Vysis filter sets are also suitable for excitation and emission of other manufacturers' FISH probes that are labeled with the common fluorophores such as rhodamine, TexasRed®, and fluorescein.

 

Vysis filter sets can be specified for most microscope types and models. Some of the most common filter holders and sliders are available from Abbott Molecular. If your model is not listed, please contact your local Abbott representative. Abbott Molecular currently does not supply the new 8 position turret for the Olympus AX-70. 

 

See the Microscope Filter Order Form for ordering information.

 

The critical components of the fluorescence microscope optical train are depicted in the diagram below.

 

fluorescence-microscope-diag-1.gif

 
 
 
 
single bandpass filter sets

 

Orange

The Orange filter set is designed to excite and transmit SpectrumOrange fluorescence. All of the Vysis SpectrumOrange labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. The SpectrumRed fluorophore will be visible using this filter set, but will be dim.

 

Red

The Red filter set is designed to excite and transmit SpectrumRed fluorescence. This filter set can be used to view, analyze, and image SpectrumRed labeled probes, or for TexasRed labeled DNA FISH probes that are available from other probe manufacturers.

 

The SpectrumRed filter set is not recommended for viewing SpectrumOrange labeled probes.

 

Yellow

The Yellow filter set is designed to excite and transmit SpectrumGold fluorescence. The Vysis SpectrumGold labeled DNA FISH probes (Vysis UroVysion) can be viewed, analyzed, and imaged using this filter set. SpectrumOrange probe fluorescence will also be visible with the Yellow filter set. SpectrumRed fluorescence may be visible, yet very dim.

 

Green

The Green filter set is designed to excite and transmit SpectrumGreen fluorescence. This filter set can be used to view, analyze, and image SpectrumGreen and fluorescence labeled DNA FISH probes.

 

Aqua

The Aqua filter set is designed to excite and transmit SpectrumAqua fluorescence. This filter set is specifically required for viewing, analyzing, and imaging Vysis SpectrumAquaTM DNA probes. Filter sets that excite and transmit DAPI fluorescence are not appropriate for SpectrumAqua labeled probes. In some instances,when hybridization signal is very intense for the SpectrumAqua labeled DNA probe, the aqua fluorescence may be visible through a DAPI filter set.

 

However, this will not provide a reliable method for analysis of SpectrumAqua labeled probes. In addition, on specimens counterstained with DAPI, extremely weak DAPI fluorescence may be observed when viewing or imaging through an Aqua single bandpass filter set.

 

Blue

The Blue filter set is designed to excite and transmit SpectrumBlue fluorescence and is useful when viewing the SpectrumBlueTM fluorophore alone. DAPI fluorescence will also be visible with this filter set. SpectrumAqua fluorescence will be visible through the Blue filter set, but will be dim. In order to view the SpectrumBlue and SpectrumAqua fluorophores simultaneously, the Aqua/Blue dual bandpass filter set should be used.

 

DAPI Longpass

The DAPI longpass filter set is designed to excite and transmit DAPI counterstain fluorescence. This filter set transmits more in the red range of the color spectrum than the DAPI bandpass filter set.

 

DAPI Bandpass

The DAPI bandpass filter set is designed to excite and transmit DAPI counterstain. This filter set does not transmit as much of the spectrum as the DAPI Longpass filter set, and is recommended over the DAPI Longpass for imaging applications.

 
 
 
 
dual bandpass filter sets

 

DAPI/Orange

The DAPI/Orange filter set is designed to excite and transmit SpectrumOrangeTM and DAPI counterstain fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA, counterstained with DAPI, and the SpectrumOrange fluorophore must be viewed concurrently. This filter set is recommended for many of the Vysis® SpectrumOrange labeled DNA FISH probes that can be analyzed simultaneously while viewing the DAPI counterstain.

 

DAPI/9-Orange (NB)

The DAPI/9-Orange (NB) filter set is designed to excite and transmit SpectrumOrange and the DAPI counterstain fluorescence simultaneously. This filter set is designed to minimize autofluorescence from paraffin-embedded specimens and is recommended for the PathVysion® HER-2 DNA Assay. This filter is useful when the nuclear and chromosomal DNA, counterstained with DAPI, and the SpectrumOrange fluorophore must be viewed concurrently.

 

DAPI/Green

The DAPI/Green filter set is designed to excite and transmit SpectrumGreen™ and DAPI fluorescence simultaneously and is recommended for the PathVysion® DNA Assay. This filter is useful when the nuclear and chromosomal DNA, counterstained with DAPI, and the SpectrumGreen fluorophore must be viewed concurrently. This filter set is recommended for many of the Vysis SpectrumGreen labeled DNA FISH probes that can be analyzed simultaneously while viewing the DAPI counterstain.

 

Blue/Aqua

The Blue/Aqua filter set is designed to excite and transmit SpectrumBlueTM and SpectrumAquaTM fluorophores simultaneously.

 
 
 
 
triple bandpass filter sets

 

DAPI/Green/Orange

The DAPI/Green/Orange filter set is designed to excite and transmit SpectrumGreen, SpectrumOrange, and DAPI counterstain fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA, counterstained with DAPI, and the two fluorophores SpectrumGreen and SpectrumOrange must be viewed concurrently. This filter set is recommended for most of the Vysis dual color probe mixtures when hybridized to specimens that are not imbedded in paraffin. Products such as CEP X/Y should be viewed using this filter set.

 

DAPI/Green/Orange (V.2)

The DAPI/Green/Orange (V.2) filter set is designed to excite and transmit SpectrumGreen, SpectrumOrange and the DAPI counterstain fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA, counterstained with DAPI, and the SpectrumGreen and SpectrumOrange fluorophores must be viewed simultaneously.

 

The DAPI/Green/Orange (V.2) filter design may provide better color distinction and brightness of the SpectrumOrange and SpectrumGreen fluorophores when viewing paraffin-embedded specimens. This filter set is not optimized for viewing dual-color translocation probes where a blending of the SpectrumGreen and SpectrumOrange fluorophores creates a yellow color. This is the recommended triple bandpass filter set for viewing and analyzing the PathVysion HER-2 DNA Assay.

 

DAPI/Green/Red

The DAPI/Green/Red filter set is designed to excite and transmit SpectrumGreen, SpectrumRed, and the DAPI counterstain fluorescence simultaneously. This filter is optimal for viewing probes labeled with SpectrumRed fluorophore while concurrently viewing SpectrumGreen and DAPI.

 
 
 
 
quad bandpass filter set

 

DAPI/Aqua/Green/Orange

The quad bandpass filter set is designed to excite and transmit DAPI, SpectrumAqua, SpectrumOrange and SpectrumGreen fluorophores simultaneously. This filter set is optimal for viewing all three probe labels simultaneously, plus the DAPI counterstain. This filter set is not recommended for viewing the AneuVysion® Assay on uncultured amniocytes.

 
 
 
 
required vysis filter set configurations

 

The following filter set configurations are required for the specific Vysis Assays, as indicated. The recommended filter sets provide the most optimal viewing conditions. If not indicated, contact your local Abbott Molecular Technical Service representative for more information on appropriate filter set configurations for imaging.

 

PathVysion HER-2 DNA Assay Filter Sets

The following filter sets are recommended for viewing and enumeration of the PathVysion HER-2 Assay. These filter sets are optimized both for Vysis SpectrumGreen and SpectrumOrange fluorophores and for paraffin-embedded specimen autofluorescence. The dual bandpass filter sets allow the user to view signals of each respective individual color fluorophore and the DAPI counterstain. The triple bandpass filter set allows the user to visualize the SpectrumGreen, SpectrumOrange, and DAPI fluorescent signals simultaneously.

 

vysis filter set fluorophores detected
DAPI/9-Orange (NB) dual bandpass SpectrumOrange and DAPI
DAPI/Green dual bandpass SpectrumGreen and DAPI
DAPI/Green/Orange (V.2) triple bandpass SpectrumGreen/SpectrumOrange/DAPI (specifically designed for viewing paraffin sections)

 

AneuVysion Assay Filter Sets

 

The following filter set configuration provides the best microscope filter set-up for viewing and analysis of the AneuVysion Assay on uncultured amniocytes.

 

VYSIS FILTER SET FLUOROPHORES DETECTED
Aqua single bandpass SpectrumAqua
Green single bandpass SpectrumGreen
Orange single bandpass SpectrumOrange
DAPI/Green/Orange triple bandpass

SpectrumGreen/SpectrumOrange/ DAPI

 

UroVysion Assay Filter Sets

 

VYSIS FILTER SET FLUOROPHORES DETECTED
DAPI single bandpass DAPI
Aqua single bandpass SpectrumAqua
Yellow single bandpass SpectrumGold
 Red/Green dual bandpass  SpectrumRed/SpectrumGreen
 
 
 
 
Indications and Limitations of Use

 

For Laboratory Use.

 
 
 
 

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