Microscope Filters

microscope filters

 

Microscope Filter Order Form »

categoryimages-fishmicro1.jpg

 

The Vysis microscope filter sets are 

high-quality filter sets that are designed to provide optimal excitation and emission of the Vysis fluorophore labeled DNA FISH probes. The specific Vysis design filter sets provide a wider bandpass, steeper profile, and maximum fluorescence throughput specific for Vysis fluorophores. In addition, the Vysis filter sets carry a one year warranty against defects.

 

Many of the Vysis filter sets are also suitable for excitation and emission of other manufacturers' FISH probes that are labeled with the common fluorophores such as rhodamine, TexasRed®, and fluorescein.

 

Vysis filter sets can be specified for most microscope types and models. Some of the most common filter holders and sliders are available from Abbott Molecular. If your model is not listed, please contact your local Abbott representative. Abbott Molecular currently does not supply the new 8 position turret for the Olympus AX-70. 

 

See the Microscope Filter Order Form for ordering information.

 

The critical components of the fluorescence microscope optical train are depicted in the diagram below.

 

fluorescence-microscope-diag-1.gif

 
 
 
 
single bandpass filter sets

 

Note: The filters listed are recommended for evaluation of Abbott Molecular Vysis FISH probes.

 

Orange

The Orange filter set is designed to excite and transmit SpectrumOrange fluorescence. The Vysis SpectrumOrange labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. The SpectrumRed fluorophore will be visible using this filter set, but will be dim.

 

Red

The Red filter set is designed to excite and transmit SpectrumRed fluorescence. The Vysis SpectrumRed labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set.  This filter set can also be used for TexasRed labeled DNA FISH probes that are available from other probe manufacturers. The SpectrumRed filter set is not recommended for viewing SpectrumOrange labeled probes.

 

Gold

The Gold filter set is designed to excite and transmit SpectrumGold fluorescence. The Vysis SpectrumGold labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. The Vysis SpectrumOrange probe fluorescence will also be visible with the Gold filter set. SpectrumRed fluorescence may be visible, yet very dim.

 

Green

The Green filter set is designed to excite and transmit SpectrumGreen fluorescence. The Vysis SpectrumGreen labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. 

 

Aqua

The Aqua filter set is designed to excite and transmit SpectrumAqua fluorescence.  The Vysis SpectrumAqua labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. Filter sets that excite and transmit DAPI fluorescence are not appropriate for SpectrumAqua labeled probes. In some instances, when a hybridization signal is very intense for the Vysis SpectrumAqua labeled DNA probe, the aqua fluorescence may be visible through a DAPI filter set.

 

However, this will not provide a reliable method for analysis of SpectrumAqua labeled probes. In addition, on specimens counterstained with DAPI, extremely weak DAPI fluorescence may be observed when viewing or imaging through an Aqua single bandpass filter set.

 

Blue

The Blue filter set is designed to excite and transmit SpectrumBlue fluorescence and is useful when viewing the SpectrumBlue fluorophore alone. The Vysis SpectrumBlue labeled DNA FISH probes can be viewed, analyzed, and imaged using this filter set. DAPI fluorescence will also be visible with this filter set. SpectrumAqua fluorescence will be visible through the Blue filter set, but will be dim. 

 

DAPI 

The DAPI filter set is designed to excite and transmit DAPI counterstain flourescence. 

 
 
 
 
dual bandpass filter sets

 

DAPI/Orange

The DAPI/Orange filter set is designed to excite and transmit SpectrumOrange and DAPI counterstain fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA is counterstained with DAPI and the SpectrumOrange fluorophore must be viewed concurrently. This filter set is recommended for many of the Vysis SpectrumOrange labeled DNA FISH probes that can be analyzed simultaneously while viewing the DAPI counterstain.

 

DAPI/9-Orange

The DAPI/9-Orange filter set is designed to excite and transmit SpectrumOrange and the DAPI counterstain fluorescence simultaneously. This filter set is designed to minimize autofluorescence from paraffin-embedded specimens. This filter is useful when the nuclear and chromosomal DNA is counterstained with DAPI and the SpectrumOrange fluorophore must be viewed concurrently.

 

DAPI/Green

The DAPI/Green filter set is designed to excite and transmit SpectrumGreen and DAPI fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA is counterstained with DAPI and the SpectrumGreen fluorophore must be viewed concurrently. This filter set is recommended for many of the Vysis SpectrumGreen labeled DNA FISH probes that can be analyzed simultaneously while viewing the DAPI counterstain.

 

 
 
 
triple bandpass filter sets

 

DAPI/Green/Orange

The DAPI/Green/Orange filter set is designed to excite and transmit SpectrumGreen, SpectrumOrange, and DAPI counterstain fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA is counterstained with DAPI and the two fluorophores SpectrumGreen and SpectrumOrange must be viewed concurrently. This filter set is recommended for most of the Vysis dual color probe mixtures when hybridized to specimens. 

 

DAPI/Green/Orange (V.2)

The DAPI/Green/Orange (V.2) filter set is designed to excite and transmit SpectrumGreen, SpectrumOrange and the DAPI counterstain fluorescence simultaneously. This filter is useful when the nuclear and chromosomal DNA is counterstained with DAPI and the SpectrumGreen and SpectrumOrange fluorophores must be viewed simultaneously.

 

The DAPI/Green/Orange (V.2) filter design may provide better color distinction and brightness of the SpectrumOrange and SpectrumGreen fluorophores when viewing paraffin-embedded specimens as compared to the DAPI/Green/Orange filter set. This filter set is not optimized for viewing dual-color translocation probes where overlay of the SpectrumGreen and SpectrumOrange fluorophores creates a yellow color. 

 

DAPI/Green/Red

The DAPI/Green/Red filter set is designed to excite and transmit SpectrumGreen, SpectrumRed, and the DAPI counterstain fluorescence simultaneously.  This filter is useful when the nuclear and chromosomal DNA is counterstained with DAPI and the SpectrumGreen and SpectrumRed fluorophores must be viewed simultaneously. This filter is optimal for viewing probes labeled with SpectrumRed fluorophore while concurrently viewing SpectrumGreen and DAPI.

 
 
 
 
required vysis filter set configurations

 

The following filter set configurations are required for the specific Vysis Assays, as indicated. The recommended filter sets provide the most optimal viewing conditions. If not indicated, contact your local Abbott Molecular Technical Service representative for more information on appropriate filter set configurations for imaging.

 

PathVysion HER-2 DNA Assay Filter Sets

The following filter sets are recommended for viewing and enumeration of the PathVysion HER-2 Assay. These filter sets are optimized both for Vysis SpectrumGreen and SpectrumOrange fluorophores and for paraffin-embedded specimen autofluorescence. The dual bandpass filter sets allow the user to view signals of each respective individual color fluorophore and the DAPI counterstain. The triple bandpass filter set allows the user to visualize the SpectrumGreen, SpectrumOrange, and DAPI fluorescent signals simultaneously.

 

vysis filter set fluorophores detected
DAPI/9-Orange (NB) dual bandpass SpectrumOrange and DAPI
DAPI/Green dual bandpass SpectrumGreen and DAPI
DAPI/Green/Orange (V.2) triple bandpass SpectrumGreen/SpectrumOrange/DAPI (specifically designed for viewing paraffin sections)

 

AneuVysion Assay Filter Sets

 

The following filter set configuration provides the best microscope filter set-up for viewing and analysis of the AneuVysion Assay on uncultured amniocytes.

 

VYSIS FILTER SET FLUOROPHORES DETECTED
Aqua single bandpass SpectrumAqua
Green single bandpass SpectrumGreen
Orange single bandpass SpectrumOrange
DAPI/Green/Orange triple bandpass

SpectrumGreen/SpectrumOrange/ DAPI

 

UroVysion Assay Filter Sets

 

VYSIS FILTER SET FLUOROPHORES DETECTED
DAPI single bandpass DAPI
Aqua single bandpass SpectrumAqua
Gold (Yellow) single bandpass SpectrumGold
 Red/Green dual bandpass  SpectrumRed/SpectrumGreen

 

CLL FISH Probe Kit Filter Sets

 

VYSIS FILTER SET   FLUOROPHORES DETECTED
 DAPI single bandpass  DAPI
 Aqua single bandpass  SpectrumAqua
 Green single bandpass  SpectrumGreen
 Orange single bandpass  SpectrumOrange
 Green/Orange dual bandpass  SpectrumGreen/SpectrumOrange
 

ALK Break Apart FISH Probe Kit Filter Sets

 

VYSIS FILTER SET   FLUOROPHORES DETECTED
 DAPI single bandpass   DAPI
  Green single bandpass  SpectrumGreen
 Orange single bandpass   SpectrumOrange
  Green/Orange dual bandpass  SpectrumGreen/SpectrumOrange
 
 
 
 
Indications and Limitations of Use

 

For Laboratory Use.

 
 
 
 

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