SPECTRAL TECHNICAL DATA 

SPECTRAL TECHNICAL DATA image
SPECTRAL TECHNICAL DATA image
SPECTRAL TECHNICAL DATA image

Vysis™ SpectrumFRed™, SpectrumRed™, SpectrumGold™, SpectrumOrange™, SpectrumGreen™, SpectrumAqua™, and SpectrumBlue™ Series Vysis'™ SpectrumFRed™, SpectrumRed™, SpectrumGold™, SpectrumOrange™, SpectrumGreen™, SpectrumAqua™, and SpectrumBlue™ Series Fluorescence in situ Hybridization (FISH)* Fluorescence in situ Hybridization (FISH)* Probes and Fluorescent Nucleotides Fluorescence Characteristics. 

VYSIS direct-labeled FISH* probes and fluor-labeled nucleotides have the following nominal fluorescence characteristics VYSIS direct-labeled FISH* probes and fluor-labeled nucleotides have the following nominal fluorescence characteristics (all units in nanometers):

 

PROBESEXCITATION PEAKEXCITATION FWHMEMISSION PEAKEMISSION FWHM
SpectrumFRed™ (far red) Probes6553967539
SpectrumRed™ Probes5923561251
SpectrumGold™ Probes5303755545
SpectrumOrange™ Probes5593858848
SpectrumGreen™1 Probes4973052456
SpectrumGreen™2 Probes5093153844
SpectrumAqua™ Probes4335348055
SpectrumBlue™ Probes4006145050
COUNTERSTAINS    
DAPI3676145292
Propium Iodide54374614 


Key:

  • FWHM = full width of the spectral band at half of the peak intensity
  • DAPI = 4', 6-diamidino-2-phenylindole

Note: Vysis' SpectrumGreen™ products may be labeled with either SpectrumGreen1 or SpectrumGreen2 fluorophore. The excitation and emission of these fluorophores are not distinguishable under normal fluorescence viewing conditions but may be spectrally distinguishable using appropriate imaging and filter configurations. Please contact your Vysis technical service representative for more information.

*U.S. Patent # 5,447,841 exclusively licensed to Vysis by the University of California covers FISH with blocking DNA and unique sequence probes such as Vysis LSI® probes. Vysis LSI, CEP® and WCP® direct label fluroescence probes are covered by U.S. patent # 5,491,224. Vysis' multiple direct label probe products are covered by U.S. patent # 5,663,319.

LSI®, CEP® and WCP® are registered trademarks of Vysis, Inc. SpectrumRed™, SpectrumOrange™, SpectrumGreen™ , SpectrumAqua™ , SpectrumBlue™, and SpectrumGold™ are trademarks of Vysis, Inc.