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Vysis™ SpectrumFRed™, SpectrumRed™, SpectrumGold™, SpectrumOrange™, SpectrumGreen™, SpectrumAqua™, and SpectrumBlue™ Series Vysis'™ SpectrumFRed™, SpectrumRed™, SpectrumGold™, SpectrumOrange™, SpectrumGreen™, SpectrumAqua™, and SpectrumBlue™ Series Fluorescence in situ Hybridization (FISH)* Fluorescence in situ Hybridization (FISH)* Probes and Fluorescent Nucleotides Fluorescence Characteristics.
VYSIS direct-labeled FISH* probes and fluor-labeled nucleotides have the following nominal fluorescence characteristics VYSIS direct-labeled FISH* probes and fluor-labeled nucleotides have the following nominal fluorescence characteristics (all units in nanometers):
Probes | Excitation Peak | Excitation FWHM | Emission Peak | Emission FWHM |
SpectrumFRed™ (far red) Probes | 655 | 39 | 675 | 39 |
SpectrumRed™ Probes | 592 | 35 | 612 | 51 |
SpectrumGold™ Probes | 530 | 37 | 555 | 45 |
SpectrumOrange™ Probes | 559 | 38 | 588 | 48 |
SpectrumGreen™1 Probes | 497 | 30 | 524 | 56 |
SpectrumGreen™2 Probes | 509 | 31 | 538 | 44 |
SpectrumAqua™ Probes | 433 | 53 | 480 | 55 |
SpectrumBlue™ Probes | 400 | 61 | 450 | 50 |
COUNTERSTAINS | ||||
DAPI | 367 | 61 | 452 | 92 |
Propium Iodide | 543 | 74 | 614 |
Key:
Note: Vysis' SpectrumGreen™ products may be labeled with either SpectrumGreen1 or SpectrumGreen2 fluorophore. The excitation and emission of these fluorophores are not distinguishable under normal fluorescence viewing conditions but may be spectrally distinguishable using appropriate imaging and filter configurations. Please contact your Vysis technical service representative for more information.
*U.S. Patent # 5,447,841 exclusively licensed to Vysis by the University of California covers FISH with blocking DNA and unique sequence probes such as Vysis LSI® probes. Vysis LSI, CEP® and WCP® direct label fluroescence probes are covered by U.S. patent # 5,491,224. Vysis' multiple direct label probe products are covered by U.S. patent # 5,663,319.
LSI®, CEP® and WCP® are registered trademarks of Vysis, Inc. SpectrumRed™, SpectrumOrange™, SpectrumGreen™ , SpectrumAqua™ , SpectrumBlue™, and SpectrumGold™ are trademarks of Vysis, Inc.
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