NORMAL

Analysis of an uncultured amniocyte (sometimes referred to as direct analysis) hybridized with the AneuVysion 18/X/Y probe set. Three aqua signals indicate three copies of chromosome 18, one green signal indicates one copy of the X chromosome and one orange signal indicates one copy of the Y chromosome.

NORMAL

Normal cell hybridization using the LSI BCL2 Dual Color Break Apart Rearrangement Probe

ABNORMAL

Abnormal cell hybridization using the LSI BCL2 Dual Color Break Apart Rearrangement Probe.

ABNORMAL

NORMAL

NORMAL

Result fo the hybridization of the LSI BIRC3/MALT1 Dual Color Dual Fusion Probes as observed in normal interphase cells.In a normal cell that lacks the t(11;18)(q21;q21) translocation, a two orange, two green signal pattern will be observed reflecting the two intact copies of MALT1 and BIRC3, respectively (Figure 1).

ABNORMAL

A cell hybridized with the LSI BIRC3/MALT1 Dual Color Dual Fusion Probe. The cell in this image shows the one orange, one green and two fusion signal pattern.

NORMAL

A cell hybridized with the LSI BIRC3/MALT1 Dual Color Dual Fusion Probe. The cell in this image shows the one orange, one green and two fusion signal pattern.

ABNORMAL

An abnormal cell hybridized with the LSI BIRC3/MALT1 Dual Color Dual Fusion Probes. The cell in this image shows the one orange, one green and two fusion signal pattern indicative of the t(11;18)(q21;q21) translocation.In an abnormal cell containing the t(11;18)(q21;q21) translocation, a one orange (MALT1), one green (BIRC3), and two fusion (BIRC3/MALT1 and MALT1/BIRC3) signal pattern will be observed. Some samples containing the t(11;18) may display signal patterns differently than the one orange, one green, and two fusions.

ABNORMAL

LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing the t(14;18) (q32;q21) showing the 1O1G2F signal pattern.The expected pattern in a normal nucleus hybridized with the LSI IGH/BCL2 probe is the two orange, two green signal pattern (2O2G). In a nucleus harboring a t(14;18), the most common pattern is one orange signal, one green signal (representing the normal homolog) and two orange/green (yellow) fusion signals representing the two derivative chromosomes resulting from the reciprocal translocation (1O1G2F pattern). Patterns other than 1O1G2F may be observed in some abnormal cells including instances of nuclei containing more than two fusion signals.

ABNORMAL

LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing the t(14;18) (q32;q21) showing the 1O1G2F signal pattern.The expected pattern in a normal nucleus hybridized with the LSI IGH/BCL2 probe is the two orange, two green signal pattern (2O2G). In a nucleus harboring a t(14;18), the most common pattern is one orange signal, one green signal (representing the normal homolog) and two orange/green (yellow) fusion signals representing the two derivative chromosomes resulting from the reciprocal translocation (1O1G2F pattern). Patterns other than 1O1G2F may be observed in some abnormal cells including instances of nuclei containing more than two fusion signals.