FISH on G-banded Slides

Dehydrate the slides at room temperature by immersing the slide in a series of ethanol wash solutions of 70%, 85%, and 100% for 2 minutes each. Allow the slide to air dry. Slides are ready for FISH. It is recommended that the slide denaturation time be decreased to approximately 50% of the original denaturation time, but not less than 3 minutes. Subsequent re-hybridization does not require further reduction of the denaturation time.

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