石蜡切片故障排除

  1. 确认 80°C 循环水浴的温度保持恒定。此温度的波动会导致较差的结果。用于表面绝缘的聚丙烯球可通过 VWR 目录编号 26396-466 购买。

    要重新处理同一张玻片:
  2. 取下盖玻片,并将载玻片依次置于一系列 (70%、85%、100%) 乙醇溶液中各 1 分钟。使载玻片干燥,并重新放回蛋白酶溶液中 10-45 分钟,具体取决于消化的水平。请勿再次对载玻片进行福尔马林固定。在 73°C 下再变性 6 分钟并在 37°C 下杂交过夜。在极端情况下,您可能需要重复从 80°C 硫氰酸钠开始直至完成蛋白酶溶液变性的步骤并再次杂交。
  3. 如果切片未经 24-48 小时的福尔马林固定,则难以达到适当的消化水平。
  4. 确认蛋白酶缓冲液的 pH 值为 2。否则,调节 pH 值以使酶将重新获得活性。
  5. 在加入蛋白酶之前,确保蛋白酶缓冲液已加热至 37°C。
  6. 在 72°C 的 2XSSC/0.3% NP-40 中洗涤石蜡切片 2 分钟。不建议在此之后进行室温洗涤。
  7. 如果预处理仍然不起作用,您可能需要切换到更极端的预处理。请联系您当地的技术服务办事处。

You are about to exit the Abbott family of websites for a 3rd party website

Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.

Do you wish to continue and exit this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No