技术提示和信息

关于如何充分利用 Vysis 产品的提示。这些可能包括使 Vysis 探针和仪器产品性能最大化的简单提示,以及在应用程序中使用 Vysis 产品的详细方案。首先在此处查看最新的技术信息。不要忘记 Vysis 常见问题;您可以在这里找到我们技术服务人员对许多经常提出问题的答案。

对照和 CGH

在执行 CGH 实验时,正确使用对照品无论怎样强调都不为过。应运行对照品以验证标记反应是否有效,还应运行其他对照品以验证杂交是否有效。此外,每次分离 DNA 时,都应该运行凝胶,以验证您尝试标记的 DNA 具有最佳片段大小且较为纯净。如果省略了这些步骤中的任何一个,并且最终结果不理想,则将很难确定到底哪里出了问题,以及在下一个实验中应该采取什么措施来纠正。获取有关正确使用控件的详细信息,请与 Vysis 技术服务部门联系。

解释批号

每个探针附带的分析证书上显示的批号即为试剂盒批号。大多数试剂盒由一小瓶探针和一小瓶杂交缓冲液组成。其他试剂盒含有与杂交缓冲液预混合的探针。每个组成部分都有唯一的部件号和批号(独立于试剂盒的部件号和批号)。从我们的目录订购产品时,您使用的编号是目录编号,而不是试剂盒中小瓶上显示的单个部件号。 

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