光谱技术数据

Vysis™ SpectrumFRed™、SpectrumRed™、SpectrumGold™、SpectrumOrange™、SpectrumGreen™、SpectrumAqua™ 和 SpectrumBlue™ 系列 Vysis'™ SpectrumFRed™、SpectrumRed™、SpectrumGold™、SpectrumOrange™、SpectrumGreen™、SpectrumAqua™ 以及 SpectrumBlue™ 系列荧光原位杂交 (FISH)* 荧光原位杂交 (FISH)* 探针以及荧光核苷酸荧光特性。 

VYSIS 直接标记 FISH* 探针和荧光标记核苷酸具有以下标称荧光特性;VYSIS 直接标记 FISH* 探针和荧光标记核苷酸具有以下标称荧光特性(所有单位均为纳米):

探针 激发峰 激发半峰全宽 发射峰 发射半峰全宽
SpectrumFRed™(远红外)探针 655 39 675 39
SpectrumRed™ 探针 592 35 612 51
SpectrumGold™ 探针 530 37 555 45
SpectrumOrange™ 探针 559 38 588 48
SpectrumGreen™1 探针 497 30 524 56
SpectrumGreen™2 探针 509 31 538 44
SpectrumAqua™ 探针 433 53 480 55
SpectrumBlue™ 探针 400 61 450 50
复染剂        
DAPI 367 61 452 92
碘化丙啶 543 74 614  

关键点:

  • FWHM = 在峰值强度一半处的光谱的全宽度
  • DAPI = 4',6-二脒基-2-苯基吲哚

注意:Vysis' SpectrumGreen™ 产品可用 SpectrumGreen1 或 SpectrumGreen2 荧光团标记。这些荧光团的激发和发射在普通荧光观察条件下不可区分,但是可以使用适当的成像和滤光片配置进行光谱区分。有关详细信息,请与您的 Vysis 技术服务代表联系。

*美国专利号 5,447,841 的美国专利由加利福尼亚大学独家授予 Vysis,该专利涉及具有封闭 DNA 和独特序列探针(如 Vysis LSI® 探针)的 FISH。Vysis LSI、CEP® 和 WCP® 直接标记荧光探针则由美国专利号 5,491,224 的美国专利涵盖。Vysis 多重直接标记探针产品则由美国专利号 5,663,319 的美国专利涵盖。

LSI®、CEP® 和 WCP® 均为 Vysis, Inc. 的注册商标。SpectrumRed™、SpectrumOrange™、SpectrumGreen™、SpectrumAqua™、SpectrumBlue™ 和 SpectrumGold™ 均为 Vysis, Inc. 的商标。

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