顺序 FISH 程序

大多数情况下,不管原因如何,如果获得的 FISH 结果不充分,可将载玻片与新的探针混合物重新杂交。如果探针是直接标记的,则可以使用相同或不同的探针。  

具体情况根据外周血液样本加以判断。

  1. 用镊子小心地从先前杂交的靶标上取下盖玻片。
  2. 将玻片在室温下顺序浸入 70%、85% 和 100% 的系列乙醇洗涤液中,并在每种浓度下脱水 1 分钟。风干玻片。玻片已经可以重新杂交。建议将玻片变性时间缩短至原始变性时间的约 50%,但不少于 3 分钟。随后的再杂交不需要进一步缩短变性时间。

复染剂强度随连续杂交而降低,然而损失最小。同一中期可与不同探针进行多达 8 次重复杂交。

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