FISH 用于 Cytospin

  • 制作离心涂片
  • 在 3:1 的甲醇:冰醋酸中固定约 20 分钟
  • 分别在 70%、85%、100% 乙醇中脱水 2 分钟
  • 风干玻片。
  • 将 40 ul RNA 酶工作液 (100ug/mL) 与 40 mL 预先加热 (37 ºC) 的 2xSSC 混合
  • 将玻片置于 37 ºC 的 RNA 酶溶液中约 15 分钟至 1 小时(取决于时间限制)
  • 用 2xSSC 冲洗玻片三次,每次两分钟
  • 分别在 70%、85%、100% 乙醇中脱水 2 分钟。室温下干燥。根据试剂盒说明书进行制备。

 如果时间不允许,可以省略 RNA 酶步骤。

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