直接骨髓涂片

 

  1. 按照您实验室的方法制备骨髓。
  2. 在室温下将载玻片或盖玻片浸入含有 70% 乙酸的 Coplin 染色缸中 30 秒。
  3. 用蒸馏水简短洗涤两次。
  4. 依次在 70%、85%、100% 无水乙醇中脱水,各 2 分钟。
  5. 浸入冷的丙酮中 2 分钟。
  6. 风干。
  7. 继续执行骨髓预处理方案。如果玻片将在当天被丢弃,则将玻片置于 73ºC 的 2XSSC 中 2 分钟。(骨髓预处理方案的步骤 1)。

*储存长达 30 个月(也许甚至更长时间)的涂片可被成功使用

 

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