用于通过 FISH 进行快速诊断的血液涂片

血液涂片是在未经处理的载玻片上制备的,即根据血液呈现的粘稠度,将 1 或 2 滴血液置于载玻片上。要将血滴涂抹开,请以一定角度持载玻片,当血液开始沿载玻片边缘形成一个平台时,将另一载玻片的末端以一定角度接触血滴并将其快速推向样本载玻片的末端。理想的血液涂片仅覆盖载玻片的一半,并且涂片的底部边缘具有羽毛状外观。对于每份样本,应该用不同浓度的血液制备两张载玻片。也要用阴性对照样本制作载玻片。载玻片标有样本名称、实验室编号和日期。竖直放置载玻片以使其干燥 10-30 分钟。

通过三次将涂片放入新鲜的 1:1 甲醇:乙酸中 2 分钟来裂解红细胞。竖直放置载玻片以使其干燥,然后在相差显微镜下检查可用细胞、细胞浓度以及用于杂交的最佳区域。淋巴细胞在相差下显示为圆形、明亮的细胞,并且是用于 FISH 分析的最佳细胞。

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