Abbott RealTime CMV

CE 标志

供体外诊断使用

Abbott RealTime CMV 是一种体外聚合酶链式反应 (PCR) 测定,用于定量测定人血浆或全血中的巨细胞病毒 (CMV) DNA。 

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描述
 

本测定旨在与临床表现和其他实验室标志物结合使用以作为开始治疗的指征,并适用于辅助监测(以血浆或全血中 DNA 水平变化来衡量的)病毒对抗病毒治疗的反应。

本测定不适于用作 CMV 筛查试验或确认是否存在 CMV 感染的诊断试验。

设计
 

开发理念

Abbott RealTime CMV 测定使用 PCR 技术结合均相实时荧光检测来定量测定 CMV DNA。针对 CMV 基因组中两个保守区域的选择性确保了对 CMV 显著而准确地检出。

单链探针设计

  • 在靶标序列不存在的情况下,探针改变其构象从而使猝灭基团足够靠近激发荧光团以便在它可以发射荧光之前吸收其能量。
  • 这些线性探针在处于“关闭”状态时会自然折叠成随机线圈。

Abbott RealTime CMV 探针设计

  • 当探针与靶标中的互补序列结合时,荧光团和猝灭基团保持分开,从而允许荧光发射和被检测到。
  • 因为在每个循环均可产生荧光,所以可以实时读取和监测 PCR 反应。

Abbott RealTime CMV 探针设计

规格
 
 
 
Abbott RealTime Cmv
灵敏度 31.2 IU/mL(20 拷贝/mL)血浆样品
62.4 IU/mL(40 拷贝/mL)全血样品
线性范围 血浆样品:31.2 IU/mL 至 1.56 亿 IU/mL
全血样品:62.4 IU/mL 至 1.56 亿 IU/mL
内部对照品 非竞争性南瓜 DNA,在提取过程中添加到裂解缓冲液
校准 外部两点校准
标准化 首个世界卫生组织用于核酸扩增技术的人巨细胞病毒国际标准 (NIBSC 09/162)
样本类型 血浆 (EDTA) 和全血 (EDTA)
输入容量 0.5 mL 血浆,0.3 mL 全血
样品制备 m2000sp - 血浆和全血
m24 sp 和手动 - 血浆

订购信息

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