Preparazione di tessuti congelati per FISH

Protocollo standard

  • Tagliare sezioni da 5 µm su vetrini silanizzati o caricati positivamente
  • Asciugare a temperatura ambiente per 15 - 30 minuti
  • Fissare in formaldeide al 4% in PBS a 0°C per 15 minuti
  • Lavare con PBS e iniziare il protocollo del kit di pretrattamento in paraffina al passaggio .2N HCl e seguire fino alla fine

Protocollo alternativo

  • Tagliare le sezioni come in precedenza e asciugare
  • Fissare in fissativo Carnoy (3:1 metanolo:acido acetico) per 5 minuti
  • Avviare il protocollo del kit di pretrattamento in paraffina appena prima del passaggio sulla proteasi (tampone di lavaggio per 5 minuti)

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