Molecular
Molecular

FISH su citocentrifugati

  • Preparazione di citocentrifugati (cytospin)
  • Fissare in 3:1 metanolo:acido acetico glaciale per ~20 minuti
  • Disidratare in soluzioni di etanolo al 70%, 85% e 100% per 2 minuti in ciascuna
  • Far asciugare i vetrini all'aria
  • Mescolare 40 ul di soluzione di lavoro di RNAsi (100 ug/ml) con 40 ml di 2x SSC preriscaldata (37°C)
  • Collocare i vetrini nella soluzione di RNAsi a 37°C per ~15 minuti - 1 ora (a seconda dei limiti di tempo)
  • Sciacquare i vetrini in 2x SSC per tre volte, due minuti per volta
  • Disidratare in soluzioni di etanolo al 70%, 85% e 100% per 2 minuti in ciascuna. Far asciugare a temperatura ambiente. Preparare in base a quanto riportato nel foglietto illustrativo.

 Il passaggio relativo alla RNAsi può essere saltato se il tempo non lo consente.

Back

You are about to exit the Abbott family of websites for a 3rd party website

Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.

Do you wish to continue and exit this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No