Striscio diretto di midollo osseo

 

  1. Preparare il midollo osseo in base alla metodologia del proprio laboratorio.
  2. Immergere i vetrini o i coprioggetti in una vaschetta Coplin contenente acido acetico al 70% per 30 secondi a temperatura ambiente.
  3. Lavare due volte, brevemente, in acqua distillata.
  4. Disidratare, in successione, in soluzioni di EtOH al 70%, 85% e 100% per 2 minuti in ciascuna.
  5. Immergere in acetone freddo per 2 minuti.
  6. Asciugare all'aria.
  7. Procedere con il protocollo di pretrattamento del midollo osseo.Se il deposito sui vetrini avviene lo stesso giorno, mettere il vetrino in 2X SSC per 2 minuti a 73ºC. (Passaggio 1 del protocollo di pretrattamento del midollo osseo).

*È possibile utilizzare con successo strisci conservati fino a 30 mesi (talvolta anche più a lungo)

 

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