Décoloration à l'hématoxyline et à l'éosine (HE)

Les étapes suivantes ne retirent pas la totalité de la coloration à l'hématoxyline et à l'éosine (HE) (qui apparaît sous forme d'autofluorescence sur la lame) ; toutefois, la plupart des lames doivent être interprétables lorsque ces étapes sont suivies.

  1. Retirez la lamelle et incubez dans une solution de prétraitement à 65 °C (thiocyanate de sodium 1M) pendant 45 minutes. Une coloration résiduelle est parfois visible. Elle sera éliminée par l'analyse de prétraitement à la paraffine.
  2. Procédez à l'analyse de prétraitement à la paraffine Vysis standard.

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