Préparation de tissus congelés pour la méthode FISH

Protocole standard

  • Réalisez des coupes de 5 µm sur des lames silanisées ou chargées positivement.
  • Laissez-les sécher à température ambiante pendant 15 à 30 min.
  • Fixez les lames dans du formaldéhyde 4 % dans du PBS à 0 °C pendant 15 min.
  • Lavez-les avec du PBS, lancez le protocole du kit de prétraitement à la paraffine à l'étape HCl 0,2N et continuez jusqu'à la fin.

Autre protocole

  • Réalisez des coupes comme dans le protocole suivant et laissez les lames sécher.
  • Fixez-les dans un fixateur de Carnoy (3:1, méthanol:acide acétique) pendant 5 min.
  • Lancez le protocole du kit de prétraitement à la paraffine juste avant l'étape de la protéase (tampon de lavage pendant 5 min).

You are about to exit the Abbott family of websites for a 3rd party website

Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.

Do you wish to continue and exit this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No