Fish sur lames de cytocentrifugation

  • Préparez les lames de cytocentrifugation.
  • Fixez dans du méthanol 3:1:acide acétique glacial pendant environ 20 minutes.
  • Déshydratez dans de l'éthanol à 70 %, 85 % puis 100 %, 2 minutes à chaque fois.
  • Laissez sécher les lames à l'air.
  • Mélangez 40 &mgr;l de solution de travail d'ARNase 100 ug/ml) avec 40 ml de solution 2X SSC préchauffée (37 °C).
  • Placez les lames dans la solution d'ARNase à 37 °C pendant environ 15 minutes à 1 heure (selon les limites de temps).
  • Rincez les lames dans une solution 2X SSC trois fois, deux minutes pour chaque rinçage.
  • Déshydratez dans de l'éthanol à 70 %, 85 % puis 100 %, 2 minutes à chaque fois. Laissez sécher à température ambiante. Préparez en fonction de la notice d'utilisation.

 L'étape ARNase peut être omise si le temps manque.

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