Eliminación de la tinción con HE

Los siguientes pasos no eliminan el 100 % de la tinción con hematoxilina-eosina (HE) (que aparece como autofluorescencia en el portaobjetos); sin embargo, la mayoría de los portaobjetos se deberían poder interpretar si se siguen estos pasos.

  1. Retire el cubreobjetos e incube en una solución de pretratamiento a 65 °C (tiocianato de sodio 1 M) durante 45 minutos. Puede que sea visible cierta tinción residual, pero se eliminará gracias al ensayo de pretratamiento de parafina.
  2. Continúe con el ensayo de pretratamiento de parafina estándar Vysis.

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