Preparación de tejido congelado para FISH

Protocolo estándar

  • Realice cortes de 5 µm en portaobjetos silanizados o con carga positiva
  • Deje secar a temperatura ambiente durante 15-30 minutos
  • Fije en formaldehído al 4 % en PBS a 0 °C durante 15 min
  • Lave con PBS e inicie el protocolo del kit de pretratamiento de parafina en el paso de HCl 0,2 N y continúe hasta el final

Protocolo alternativo

  • Realice los cortes como antes y déjelos secar
  • Fije en fijador de Carnoy (metanol:ácido acético, 3:1) durante 5 minutos
  • Comience el protocolo del kit de pretratamiento de parafina justo antes del paso de la proteasa (tampón de lavado durante 5 minutos)

You are about to exit the Abbott family of websites for a 3rd party website

Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.

Do you wish to continue and exit this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No

You are about to enter an Abbott country or region specific website.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

Do you wish to continue and enter this website?

Yes No