FISH en preparaciones de Cytospin

  • Realice las preparaciones de Cytospin.
  • Fije en metanol:ácido acético glacial 3:1 durante unos 20 minutos.
  • Deshidrate en etanol al 70 %, 85 % y 100 % durante 2 minutos cada uno.
  • Deje los portaobjetos secar al aire.
  • Mezcle 40 µl de solución de trabajo de ARNasa (100 µg/ml) con 40 ml precalentados (37 °C) de 2xSSC.
  • Coloque los portaobjetos en la solución de ARNasa a 37 °C durante unos 15 minutos a 1 hora (dependiendo de las limitaciones de tiempo).
  • Lave los portaobjetos en 2xSSC tres veces, dos minutos cada uno.
  • Deshidrate en etanol al 70 %, 85 % y 100 % durante 2 minutos cada uno. Deje secar a temperatura ambiente. Prepare según el prospecto.

 El paso de la ARNasa puede omitirse si no se dispone de suficiente tiempo.

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