Frotis directos de médula ósea

 

  1. Prepare la médula ósea de acuerdo con la metodología de su laboratorio.
  2. Sumerja los portaobjetos o los cubreobjetos en una cubeta tipo Coplin con ácido acético al 70 % durante 30 segundos a temperatura ambiente.
  3. Lave dos veces, brevemente, en agua destilada.
  4. Deshidrátelos sucesivamente en etanol al 70 %, 85 % y 100 % durante 2 minutos cada uno.
  5. Sumérjalos en acetona fría durante 2 minutos.
  6. Déjelos secar al aire.
  7. Continúe con el protocolo de pretratamiento de médula ósea. Si los portaobjetos se van a procesar el mismo día, ponga el portaobjetos en 2X SSC durante 2 minutos a 73 °C (paso 1 de protocolo de pretratamiento de médula ósea).

*Los frotis conservados hasta 30 meses (quizás incluso más) se pueden utilizar con buenos resultados.

 

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